TRANSIENT EXPRESSION OF GUS IN ONION TRANSFORMANTS
نویسندگان
چکیده
منابع مشابه
Transient GUS Expression in Decapitated Lentil Embryos
Lentil (Lens culinaris Medik.) is an important proteinand carbohydrate-rich food for many developing countries and is also popular in developed countries where they are perceived as a healthy component of the diet. Lentil seeds contain about 24.2% high quality protein, 60% carbohydrates and 2.4 4.2% mineral matter (Hulse 1994). Although lentil is one of the oldest cultivated crop plants, its ag...
متن کاملSeasonal changes in the transient expression of a 35S CaMV-GUS
ß-glucuronidase gene (GUS) driven by a constitutive 35S CaMV-promoter in Scots pine (Pinus sylvestris L.) buds were studied by the microprojectile DNA-delivery method. Buds were collected from 5-, 15and 50-year-old trees. In buds from all age groups the amount of transient expression was dependent on the season; the highest values were found in March, and values were lowest both at the beginnin...
متن کاملAgrobacterium-mediated transient GUS gene expression in buffel grass (Cenchrus ciliaris L.).
The study was conducted to standardize a protocol for Agrobacterium-mediated genetic transformation of buffel grass (Cenchrus ciliaris L.). Embryogenic calli, produced from one-year-old mature seeds of buffel grass, were used as target cells for Agrobacterium-mediated transformation. A. tumefaciens strain LBA4404, harbouring pCAMBIA-1301 or pCAMBIA-2301, was used for co-cultivation with embryog...
متن کاملIdentifying subcellular protein localization with fluorescent protein fusions after transient expression in onion epidermal cells.
Most biochemical functions of plant cells are carried out by proteins which act at very specific places within these cells, for example, within different organelles. Identifying the subcellular localization of proteins is therefore a useful tool to narrow down the possible functions that a novel or unknown protein may carry out. The discovery of genetically encoded fluorescent markers has made ...
متن کاملSeasonal changes in the transient expression of a 35S CaMV-GUS gene construct introduced into Scots pine buds.
Seasonal changes in the transient expression of Beta-glucuronidase gene (GUS) driven by a constitutive 35S CaMV-promoter in Scots pine (Pinus sylvestris L.) buds were studied by the microprojectile DNA-delivery method. Buds were collected from 5-, 15- and 50-year-old trees. In buds from all age groups the amount of transient expression was dependent on the season; the highest values were found ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: HortScience
سال: 1996
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.31.5.759b